Immobilization of organophosphorus hydrolase for the degradation of organophosphorus nerve agents.
نویسندگان
چکیده
منابع مشابه
Specific adhesion to cellulose and hydrolysis of organophosphate nerve agents by a genetically engineered Escherichia coli strain with a surface-expressed cellulose-binding domain and organophosphorus hydrolase.
A genetically engineered Escherichia coli cell expressing both organophosphorus hydrolase (OPH) and a cellulose-binding domain (CBD) on the cell surface was constructed, enabling the simultaneous hydrolysis of organophosphate nerve agents and immobilization via specific adsorption to cellulose. OPH was displayed on the cell surface by use of the truncated ice nucleation protein (INPNC) fusion s...
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Organophosphorus compounds are widely used in pesticides, insecticides in agriculture and as nervous chemical agents. These chemicals inhibit the acetylcholinesterase enzyme activity that is responsible for the nervous impulse in organisms. This effect leads to an increase in acetylcholine level and finally neuronal complications. Many methods are used to degrade and decontaminate these compou...
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A new biometallization route for detecting low levels of organophosphorus nerve agents based on organophosphorus-hydrolase (OPH)-stimulated formation of cupric-ferrocyanide (CuFeCN) nanoparticles is described. The growth and accumulation of these nanoparticles onto the surface of carbon paste electrodes, coupled with their favorable redox activity, allow the amplified electrochemical detection ...
متن کاملImproved degradation of organophosphorus nerve agents and p-nitrophenol by Pseudomonas putida JS444 with surface-expressed organophosphorus hydrolase.
Pseudomonas putida JS444, isolated from p-nitrophenol (PNP) contaminated waste sites, was genetically engineered to simultaneously degrade organophosphorus pesticides (OP) and PNP. A surface anchor system derived from the ice-nucleation protein (INP) from Pseudomonas syringae was used to target the organophosphorus hydrolase (OPH) onto the surface of Pseudomonas putida JS444, reducing the poten...
متن کاملDetoxification of organophosphate nerve agents by immobilized Escherichia coli with surface-expressed organophosphorus hydrolase.
An improved whole-cell technology for detoxifying organophosphate nerve agents was recently developed based on genetically engineered Escherichia coli with organophosphorus hydrolase anchored on the surface. This article reports the immobilization of these novel biocatalysts on nonwoven polypropylene fabric and their applications in detoxifying contaminated wastewaters. The best cell loading (2...
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عنوان ژورنال:
- The Journal of general and applied microbiology
دوره 62 1 شماره
صفحات -
تاریخ انتشار 2016